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1Interdepartment of Physiology, Chulalongkorn University Graduate School, Bangkok, Thailand.
2Department of Medicine, Lerdsin General Hospital, Bangkok, Thailand.
3Division of Nephrology, Department of Medicine, Chulalongkorn University Faculty of Medicine, Bangkok, Thailand.
4Department of Physiology, Chulalongkorn University Faculty of Medicine, Bangkok, Thailand.
Copyright © 2019 Korean Endocrine Society
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
CONFLICTS OF INTEREST: No potential conflict of interest relevant to this article was reported.
AUTHOR CONTRIBUTIONS:
Staining intensity: 0, negative, no reactivity; 1, trace, faint or pale brown staining with less membrane reactivity; 2, weak, light brown staining with incomplete membrane reactivity; 3, moderate, shaded of brown staining of intermediate darkness with usually almost complete membrane reactivity; 4, strong, dark brown to black staining with a usually complete membrane pattern, producing a thick outline of the cell [171819].
Aldo, aldosterone; Ep., eplerenone (n=6/group); GL, glomerulus; PCT, proximal convoluted tubule; DCT, distal convoluted tubule; CCD, cortical collecting duct; Pcap, peritubular capillary; TALH, thick ascending limb of the loop of Henle; MCD, medullary collecting duct; VR, vasa recta; tLH, thin limb of the loop of Henle.
Aldo, aldosterone; Ep., eplerenone (n=6/group); GL, glomerulus; PCT, proximal convoluted tubule; DCT, distal convoluted tubule; CCD, cortical collecting duct; Pcap, peritubular capillary; TALH, thick ascending limb of the loop of Henle; MCD, medullary collecting duct; VR, vasa recta; tLH, thin limb of the loop of Henle; NS, non-significant (n=6/group).
aP<0.05.
Variable | Sham | Aldo | Ep.+Aldo |
---|---|---|---|
Plasma aldosterone, pmol/L | 1,315.11±10.22 | 6,234.33±92.88a | 6,288.55±87.47a |
Plasma sodium, mmol/L | 141.32±1.48 | 142.17±6.32 | 140.22±4.82 |
Plasma potassium, mmol/L | 3.53±0.22 | 3.58±0.26 | 3.49±0.28 |
Plasma chloride, mmol/L | 102.64±1.34 | 102.31±2.91 | 102.42±2.62 |
Plasma bicarbonate, mmol/L | 24.71±1.14 | 24.29±1.58 | 24.48±1.64 |
Plasma creatinine, mg/dL | 0.24±0.02 | 0.24±0.02 | 0.24±0.03 |
Blood urea nitrogen, mg/dL | 19.22±1.64 | 20.48±3.28 | 20.25±2.68 |
Ratio of plasma sodium to potassium | 41.27±3.64 | 39.87±2.94 | 40.38±2.87 |
Ratio of urinary sodium to potassium | 0.32±0.02 | 0.32±0.02 | 0.32±0.03 |
Striatin | Caveolin-1 | |||||
---|---|---|---|---|---|---|
Sham | Aldo | Ep.+Aldo | Sham | Aldo | Ep.+Aldo | |
Cortex | ||||||
GL | 1 | 3 | 2 | 1 | 4 | 3 |
PCT | 1 | 2 | 2 | 0 | 1 | 1 |
DCT | 1 | 2 | 2 | 0 | 1 | 1 |
CCD | 2 | 3 | 2 | 0 | 1 | 1 |
Pcap | 1 | 3 | 3 | 3 | 4 | 4 |
Outer medulla | ||||||
TALH | 2 | 2 | 3 | 1 | 2 | 1 |
MCD | 2 | 2 | 4 | 1 | 2 | 1 |
VR | 3 | 4 | 4 | 4 | 4 | 4 |
tLH | 3 | 3 | 4 | 0 | 2 | 1 |
Inner medulla | ||||||
MCD | 3 | 4 | 4 | 1 | 1 | 1 |
VR | 3 | 4 | 4 | 2 | 4 | 4 |
tLH | 2 | 3 | 4 | 2 | 1 | 2 |
Areas | Mean rank | P value | ||
---|---|---|---|---|
Sham | Aldo | Ep.+Aldo | ||
Striatin | ||||
Cortex | ||||
GL | 3 | 12.6 | 8.4 | 0.002a |
PCT | 4.2 | 10.6 | 9.2 | 0.029a |
DCT | 3 | 10 | 11 | 0.005a |
CCD | 5.8 | 12.4 | 5.8 | 0.011a |
Pcap | 3 | 10.5 | 10.5 | 0.004a |
Outer medulla | ||||
TALH | 5.9 | 5.9 | 12.2 | 0.015a |
MCD | 5.5 | 5.5 | 13 | 0.004a |
VR | 5 | 9.5 | 9.5 | 0.024a |
tLH | 5 | 7.4 | 11.6 | 0.024a |
Inner medulla | ||||
MCD | 3.8 | 10.1 | 10.1 | 0.015a |
VR | 5 | 9.5 | 9.5 | 0.001a |
tLH | 3 | 9 | 12 | 0.003a |
Cav-1 | ||||
Cortex | ||||
GL | 3 | 12.6 | 8.5 | 0.002a |
PCT | 3 | 10.5 | 10.5 | 0.001a |
DCT | 3 | 10.5 | 10.5 | 0.001a |
CCD | 3 | 10.5 | 10.5 | 0.001a |
Pcap | 3 | 10.5 | 10.5 | 0.001a |
Outer medulla | ||||
TALH | 5.5 | 13 | 5.5 | 0.001a |
MCD | 5.5 | 13 | 5.5 | 0.001a |
VR | 8 | 8 | 8 | 1.000 (NS) |
tLH | 3 | 13 | 8 | 0.001a |
Inner medulla | ||||
MCD | 8 | 8 | 8 | 1.000 (NS) |
VR | 3 | 10.5 | 10.5 | 0.001a |
tLH | 13 | 5.5 | 5.5 | 0.001a |
Values are expressed as mean±standard deviation. Aldo, aldosterone; Ep., eplerenone ( a
Staining intensity: 0, negative, no reactivity; 1, trace, faint or pale brown staining with less membrane reactivity; 2, weak, light brown staining with incomplete membrane reactivity; 3, moderate, shaded of brown staining of intermediate darkness with usually almost complete membrane reactivity; 4, strong, dark brown to black staining with a usually complete membrane pattern, producing a thick outline of the cell [ Aldo, aldosterone; Ep., eplerenone (
Aldo, aldosterone; Ep., eplerenone ( a