Fig. 1Distribution of Preso1-IR in the adult forebrain. (A-E) In the parasagittal view, Nestin (red)-expressing neuroblasts in the rostral migratory stream (RMS) are colabeled with Preso1 (green). (F-H) In the coronal section of the RMS, doublecortin (DCX)-expressing neuroblasts (red) also exhibited immunoreactivity for Preso1 (Preso1-IR; green). (I-K) In the subependymal layer (SEL) of the olfactory bulb (OB), NeuN-negative migrating neuroblasts strongly exhibited Preso1-IR, but Preso1-IR was markedly reduced in mature NeuN-expressing neurons (red) in the granule cell layer (GCL). Nuclei were counterstained with Hoechst33342 (blue). The scale bar in B=500 µm, in E, H, K=20 µm. LV, lateral ventricle.
Fig. 2Immunoreactivity for Preso1 (Preso1-IR) in the subventricular zone (SVZ) of the adult mouse. (A-C) The majority of glial fibrillary acidic protein (GFAP)-expressing astrocytes (red) and stem cells did not exhibit Preso1-IR (green). (D-F) A few Nestin-expressing transit amplifying stem cells (red) exhibited Preso1-IR (green). (G-I) The majority of doublecortin (DCX)-expressing neuroblasts (red) also exhibited strong Preso1-IR (green). (J) Schematic diagram of the Preso1-expressing stage (green) of adult neural stem cells (NSCs) in the SVZ. The scale bar in I=10 µm, enlarged inset in I=5 µm. TAC, transit-amplifying cell.
Fig. 3Polarized distributions of immunoreactivity for Preso1 (Preso1-IR) in cultured neural stem cells (NSCs). (A-C) NSCs were labeled with Preso1IR (green), F-actin (red), and nuclei (blue) were counterstained with rhodamine-phalloidin and Hoechst33342, respectively. Preso1-IR was unequally distributed in the cell cytosol. (D-F) NSCs migrating out from the neurosphere cores were stained with Preso1 (green) and GM130 (red), a Golgi apparatus marker. Preso1-IR was evenly distributed regardless of the cell polarity in migrating NSCs.
Fig. 4Distribution of immunoreactivity for Preso1 (Preso1-IR) in the subgranular zone (SGZ) of the hippocampal formation. (A-C) Preso1-IR (green) was found throughout the adult hippocampus, while Nestin (red) was expressed in the SGZ. (D-F) In the SGZ, Preso1-IR was strongly expressed in the nuclei of a subset of cells. Cells with nuclear Preso1-IR did not express Nestin (red). (G-L) Most doublecortin (DCX)-expressing neuroblasts (red) exhibited nuclear Preso1-IR (green). Nuclei were counter-stained with Hoechst33342 (blue). The scale bar in F=5 µm, I=50 µm.
Fig. 5Polarized Preso1 expression during asymmetric cell division. (A-C) Tuj-1 (red) expressing neuronal daughter cell-expressed Trim32 (green) in cultured neural stem cells (NSCs). (D-G) In the subgranular zone (SGZ), Trim32 (red)-expressing daughter cells also expressed nuclear Preso1-IR (green). (H) Schematic diagram of the Preso1 expression stages of the adult NSCs of the SGZ. RGL, radial glia-like; IPCs, intermediate precursor cells; GFAP, glial fibrillary acidic protein; DCX, doublecortin.